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Publications: PROF Richard Pickersgill

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Juodeikis R, Ulrich R, Clarke C, Banasik M, Deery E, Saalbach G, Kräutler B, Carding SR et al. ( 2025 ) . Extracellular vesicle-linked vitamin B12 acquisition via novel binding proteins in Bacteroides thetaiotaomicron . Biochemical Journal vol. 482 , ( 23 ) 1793 - 1812 .
Gu S, Bradley-Clarke J, Rose R-S, Warren MJ, Pickersgill RW ( 2024 ) . Enzyme-cargo encapsulation peptides bind between tessellating tiles of the bacterial microcompartment shell . Journal of Biological Chemistry vol. 300 , ( 6 )
Zhang S, Gu S, Rycroft P, Ruaudel F, Delolme F, Ballut L ( 2022 ) . Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall . mBio vol. 13 , ( 3 ) e00253 - e00222 .
Robledo Retana T, Bradley-Clarke J, Croll T, Rose R, Stagg AJ, Villalba M, Honti I, Pickersgill RW ( 2020 ) . Lig v 1 structure and the inflammatory response to the Ole e 1 protein family . Allergy
Conti D, Gul P, Islam A, Martín-Durán JM ( 2019 ) . Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation . eLife vol. 8 ,
Lee MJ, Mantell J, Brown IR, Fletcher JM, Verkade P, Woolfson DN ( 2018 ) . De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments . Nature Communications vol. 9 , ( 1 )
Pluta P, Roversi P, Bernardo-Seisdedos G, Rojas AL, Cooper JB, Gu S ( 2018 ) . Structural basis of pyrrole polymerization in human porphobilinogen deaminase . Biochimica et Biophysica Acta (BBA) - General Subjects vol. 1862 , ( 9 ) 1948 - 1955 .
Uddin I, Frank S, Pickersgill RW ( 2018 ) . A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes . Small vol. 14 , ( 19 )
Uddin I, Frank S, Warren MJ ( 2018 ) . Synthetic Protein Nanotubes: A Generic Self‐Assembly Process in Microcompartments and Synthetic Protein Nanotubes (Small 19/2018) . Small vol. 14 , ( 19 )
Damavandi PT, Dove MT ( 2017 ) . A review of drug therapy for sporadic fatal insomnia . Prion vol. 11 , ( 5 ) 293 - 299 .
Consortium TC ( 2017 ) . Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes . Glycobiology vol. 28 , ( 1 ) 3 - 8 .
Gu S, Shevchik VE, Shaw R, Pickersgill RW ( 2017 ) . The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics vol. 1865 , ( 10 ) 1255 - 1266 .
Qui J, Zeng Y, Pickersgill R, Sushko O ( 2017 ) . Introducing Quasi-Optical Terahertz Circular Dichroism Spectroscopy . Conference: 2017 XI International Conference on Antenna Theory and Techniques (ICATT)26 - 29 .
Gu S, Sushko O, Deery E ( 2015 ) . Crystal structure of CobK reveals strand-swapping between Rossmann-fold domains and molecular basis of the reduced precorrin product trap . Scientific Reports vol. 5 , ( 1 )
Ali S, Teixeira S, Pickersgill RW ( 2015 ) . Structural insights into the loss of catalytic competence in pectate lyase activity at low pH . FEBS Letters vol. 589 , ( 21 ) 3242 - 3246 .
Sushko O, Qiu J, Dubrovka R ( 2015 ) . Sub-Terahertz Spectroscopy as a Probe for Protein Stability in an Ionic Environment . Conference: 2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz)1 - 2 .
Pang A, Frank S, Warren MJ ( 2014 ) . Structural insights into higher order assembly and function of the bacterial microcompartment protein PduA . J Biol Chem vol. 289 , ( 32 ) 22377 - 22384 .
Lawrence AD, Taylor SL, Scott A, Rowe ML, Johnson CM, Rigby SEJ, Geeves MA ( 2014 ) . FAD binding, cobinamide binding and active site communication in the corrin reductase (CobR) . Biosci Rep vol. 34 , ( 4 )
Palmer DJ, Schroeder S, Deery E, Lobo SA, Saraiva LM, McLean KJ, Ferguson SJ, Pickersgill RW et al. ( 2014 ) . The structure, function and properties of sirohaem decarboxylase - an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway . MOLECULAR MICROBIOLOGY vol. 93 , ( 2 ) 247 - 261 .
Terali K, Beavil RL, Pickersgill RW ( 2013 ) . The effect of the adaptor protein Isd11 on the quaternary structure of the eukaryotic cysteine desulphurase Nfs1 . Biochem Biophys Res Commun vol. 440 , ( 2 ) 235 - 240 .
Gu S, Rehman S ( 2013 ) . Crystal structure of the bacterial type II secretion system secretin/pilotin complex . Acta Crystallographica Section A: Foundations and advances . vol. 69 , s351 - s351 .
Rehman S, Gu S, Shevchik VE ( 2013 ) . Anatomy of secretin binding to the Dickeya dadantii type II secretion system pilotin . Acta Crystallogr D Biol Crystallogr vol. 69 , ( Pt 8 ) 1381 - 1386 .
McIntosh AI, Yang B, Goldup SM, Watkinson M ( 2013 ) . Crystallization of amorphous lactose at high humidity studied by terahertz time domain spectroscopy . Chemical Physics Letters vol. 558 , 104 - 108 .
Pang A, Liang M, Prentice MB, Pickersgill RW ( 2012 ) . Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB . Acta Crystallogr D Biol Crystallogr vol. 68 , ( Pt 12 ) 1642 - 1652 .
Ali S, Blakeley M, Søndergaard CR, Pellegrini E ( 2012 ) . A joint neutron/X-ray crystallographic study on the mechanism of pectate lyase . Acta Crystallographica Section A: Foundations and advances . vol. 68 , s91 - s91 .
Gu S, Kelly G, Wang X, Shevchik VE ( 2012 ) . Solution structure of homology region (HR) domain of type II secretion system . J Biol Chem vol. 287 , ( 12 ) 9072 - 9080 .
Gu S, Rehman S, Wang X, Shevchik VE ( 2012 ) . Structural and functional insights into the pilotin-secretin complex of the type II secretion system . PLoS Pathog vol. 8 , ( 2 )
Deery E, Schroeder S, Lawrence AD, Taylor SL, Seyedarabi A, Brown D, Geeves MA, Howard MJ ( 2012 ) . An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis . NATURE CHEMICAL BIOLOGY vol. 8 , ( 11 ) 933 - 940 .
Wang X, Gu S, Guschinskaya N, Pickersgill RW ( 2012 ) . Cysteine Scanning Mutagenesis and Disulfide Mapping Analysis of Arrangement of GspC and GspD Protomers within the Type 2 Secretion System . JOURNAL OF BIOLOGICAL CHEMISTRY vol. 287 , ( 23 ) 19082 - 19093 .
Martin JE, Chowdhury ATMD, McElwaine S, Fikree A, Minassian B, Broad J, Knowles CH, Allen L et al. ( 2012 ) . Intestinal Glycogen Storage Disease, and the Molecular Basis of Pseudo-obstruction with Polyglucosan Bodies . JOURNAL OF PATHOLOGY . vol. 226 , S24 - S24 .
Pickersgill RW ( 2012 ) . Pathology: A Structural Biologist's View . JOURNAL OF PATHOLOGY . vol. 226 , S7 - S7 .
Ali S, Texeira S ( 2011 ) . Joint neutron/X-ray crystallographic study on the mechanism of pectate lyase . Acta Crystallographica Section A: Foundations and advances . vol. 67 , c734 - c734 .
Martin JE, Chowdhury AH, McElwaine S, Fikree A, Broad J, Knowles CH, Allen L, Bevan S et al. ( 2011 ) . A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease . Gastroenterology vol. 140 , ( 5 ) s - 705 .
Pang A, Warren MJ ( 2011 ) . Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe-4S cluster-binding site . Acta Crystallogr D Biol Crystallogr vol. 67 , ( Pt 2 ) 91 - 96 .
Romao CV, Ladakis D, Lobo SAL, Carrondo MA, Brindley AA, Deery E ( 2011 ) . Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization . P NATL ACAD SCI USA vol. 108 , ( 1 ) 97 - 102 .
Martin JE, Chowdhury AH, McElwaine S, Fikree A, Broad J, Knowles CH, Allen L, Bevan S et al. ( 2011 ) . A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease . GASTROENTEROLOGY . vol. 140 , S705 - S705 .
Brocklehurst K, Gul S ( 2010 ) . Substrate Recognition . Amino Acids, Peptides and Proteins in Organic Chemistry , Wiley
Seyedarabi A, Hutchison T, To TT, Deery E, Brindley A ( 2010 ) . Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus . Acta Crystallogr Sect F Struct Biol Cryst Commun vol. 66 , ( Pt 12 ) 1652 - 1656 .
Seyedarabi A, Sullivan JA, Pickersgill RW ( 2010 ) . A disulfide driven domain swap switches off the activity of Shigella IpaH9.8 E3 ligase . FEBS Lett vol. 584 , ( 19 ) 4163 - 4168 .
Shala K, Yang B, TT T, Pickersgill RW, Krauss N ( 2010 ) . Millimeter wave spectroscopy of glucose and a calcium-binding protein using a vector network analyser . Conference: 35th IRMMW-THZ Internation Conference, 2010 ( Rome, Italy ) from: 05/09/2010 to: 10/09/2010 , 1 - 2 .
Login FH, Fries M, Wang XH, Pickersgill RW ( 2010 ) . A 20-residue peptide of the inner membrane protein OutC mediates interaction with two distinct sites of the outer membrane secretin OutD and is essential for the functional type II secretion system in Erwinia chrysanthemi . Molecular Microbiology vol. 76 , ( 4 ) 944 - 955 .
Seyedarabi A, To TT, Ali S, Fries M, Madsen R, Clausen MH, Brocklehurst K ( 2010 ) . Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase . Biochemistry vol. 49 , ( 3 ) 539 - 546 .
Heldt D, Frank S, Seyedarabi A, Ladikis D, Warren MJ ( 2009 ) . Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri . BIOCHEM J vol. 423 , 199 - 207 .
Brocklehurst K, Gul S, Pickersgill RW ( 2009 ) . Substrate Recognition . Amino Acids Peptides and Proteins in Organic Chemistry Volume 2 Modified Amino Acids Organocatalysis and Enzymes , vol. 2 ,
Brindley AA, Pickersgill RW, Partridge JC, Dunstan DJ, Hunt DM, Warren MJ ( 2008 ) . Enzyme sequence and its relationship to hyperbaric stability of artificial and natural fish lactate dehydrogenases . PLoS One vol. 3 , ( 4 )
Lawrence AD, Deery E, McLean KJ, Munro AW, Rigby SEJ ( 2008 ) . Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis . J BIOL CHEM vol. 283 , ( 16 ) 10813 - 10821 .
Fries M, Ihrig J, Brocklehurst K, Pickersgill RW ( 2007 ) . Molecular basis of the activity of the phytopathogen pectin methylesterase . EMBO J vol. 26 , ( 17 ) 3879 - 3887 .
Frank S, Deery E, Brindley AA, Leech HK, Lawrence A, Heathcote P, Brocklehurst K ( 2007 ) . Elucidation of substrate specificity in the cobalamin (vitamin B12) biosynthetic methyltransferases. Structure and function of the C20 methyltransferase (CbiL) from Methanothermobacter thermautotrophicus . J Biol Chem vol. 282 , ( 33 ) 23957 - 23969 .
Pickersgill RW ( 2007 ) . Design and engineering of hydrolytic enzymes for enhanced performance in the agricultural and food industries . Journal of Chemical Technology & Biotechnology vol. 57 , ( 3 ) 290 - 291 .
Ooi A, Hussain S, Seyedarabi A ( 2006 ) . Structure of internalin C from Listeria monocytogenes . Acta Crystallogr D Biol Crystallogr vol. 62 , ( Pt 11 ) 1287 - 1293 .
Opaleye O, Rose R-S, Whittaker MM, Woo E-J, Whittaker JW, Pickersgill RW ( 2006 ) . Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase . J Biol Chem vol. 281 , ( 10 ) 6428 - 6433 .
Graham CE, Pickersgill RW, Warren MJ ( 2006 ) . Characterization of retinaldehyde dehydrogenase 3 . Biochem J vol. 394 , ( Pt 1 ) 67 - 75 .
Frank S, Brindley AA, Deery E, Heathcote P, Lawrence AD, Pickersgill RW ( 2005 ) . Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway . Biochem Soc Trans vol. 33 , ( Pt 4 ) 811 - 814 .
Okuda J, Toyotome T, Kataoka N, Ohno M, Shimura Y, Pickersgill R ( 2005 ) . Shigella effector IpaH9.8 binds to a splicing factor U2AF(35) to modulate host immune responses . BIOCHEM BIOPH RES CO vol. 333 , ( 2 ) 531 - 539 .
SUMNER IG, TAYLOR MAJ, PICKERSGILL RW ( 2005 ) . Factors effecting the thermostability of cysteine proteinases from Carica papaya . The FEBS Journal vol. 214 , ( 1 ) 129 - 134 .
PICKERSGILL RW, SUMNER IG ( 2005 ) . Preliminary crystallographic data for protease Ω . The FEBS Journal vol. 190 , ( 2 ) 443 - 444 .
Jenkins J, Hugouvieux-Cotte-Pattat N, Pickersgill RW ( 2004 ) . The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi . J BIOL CHEM vol. 279 , ( 10 ) 9139 - 9145 .
Pickersgill RW ( 2003 ) . A primordial structure underlying amyloid . STRUCTURE vol. 11 , ( 2 ) 137 - 138 .
PICKERSGILL RW, Voragen AGJ, Voragen F, Schols H, Visser R ( 2003 ) . The structures and active sites of pectinases . Advances in pectin and pectinase research . Conference: International symposium on pectins and pectinases (2nd) ( Rotterdam, The Netherlands ) from: 05/2001 to: 05/2001 , 267 - 278 .
Pickersgill RW ( 2002 ) . Complex cell signaling molecules from ancient molecular glue . STRUCTURE vol. 10 , ( 10 ) 1287 - 1288 .
Woo EJ, Marshall J, Bauly J, Venis M, Napier RM ( 2002 ) . Crystal structure of auxin-binding protein 1 in complex with auxin . EMBO J vol. 21 , ( 12 ) 2877 - 2885 .
Loris R, Steyaert J, Maes D, Lisgarten J ( 2002 ) . Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin . Biochemistry vol. 32 , ( 34 ) 8772 - 8781 .
Bhat MK, Pickersgill RW, Perry BN, Brown RA, Jones ST, Mueller-Harvey I ( 2002 ) . Modification of the head-group selectivity of porcine pancreatic phospholipase A2 by protein engineering . Biochemistry vol. 32 , ( 45 ) 12203 - 12208 .
Palomäki T, Pickersgill R, Riekki R ( 2002 ) . A putative three-dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora . Mol Microbiol vol. 43 , ( 3 ) 585 - 596 .
Pickersgill RW, Sumner IG, Collins ME, Warwicker J, Bhat KM ( 2001 ) . Modification of the stability of phospholipase A2 by charge engineering . FEBS Letters vol. 281 , ( 1-2 ) 219 - 222 .
Lo Leggio L, Kalogiannis S, Eckert K, Bhat MK, Andrei C, Pickersgill RW ( 2001 ) . Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A . FEBS Lett vol. 509 , ( 2 ) 303 - 308 .
Pickersgill R, Varvill K, Jones S, Fischer B, Henderson I, Garrard S ( 2001 ) . Making a small enzyme smaller; removing the conserved loop structure of hen lysozyme . FEBS Letters vol. 347 , ( 2-3 ) 199 - 202 .
Jenkins J ( 2001 ) . The architecture of parallel beta-helices and related folds . PROG BIOPHYS MOL BIO vol. 77 , ( 2 ) 111 - 175 .
Hogg D, Woo EJ, Bolam DN, McKie VA, Pickersgill RW ( 2001 ) . Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding . J Biol Chem vol. 276 , ( 33 ) 31186 - 31192 .
Teixeira S, Lo Leggio L ( 2001 ) . Anisotropic refinement of the structure of Thermoascus aurantiacus xylanase I . ACTA CRYSTALLOGR D vol. 57 , 385 - 392 .
Jenkins J, Mayans O, Pickersgill RW ( 2001 ) . Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site . J Mol Biol vol. 305 , ( 4 ) 951 - 960 .
Woo E-J, Bauly J, Chen J-G, Marshall J, Macdonald H, Goodenough P, Pickersgill R ( 2000 ) . Crystallization and preliminary X-ray analysis of the auxin receptor ABP1 . Acta Crystallographica Section D, Structural Biology vol. 56 , ( 11 ) 1476 - 1478 .
Woo E-J, Dunwell JM, Goodenough PW, Marvier AC ( 2000 ) . Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities . Nature Structural & Molecular Biology vol. 7 , ( 11 ) 1036 - 1040 .
Jenkins J, Leggio LL, Harris G ( 2000 ) . β‐Glucosidase, β‐galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes wit 8‐fold β/α architecture and with two conserved glutamates near the carboxy‐terminal ends of β‐strands four and seven . FEBS Letters vol. 362 , ( 3 ) 281 - 285 .
Smyth MG, Lax AJ ( 2000 ) . The potent mitogen Pasteurella multocida toxin is highly resistant to proteolysis but becomes susceptible at lysosomal pH . FEBS Letters vol. 360 , ( 1 ) 62 - 66 .
Leggio LL, Jenkins J, Pickersgill RW ( 2000 ) . X‐ray crystallographic study of xylopentaose binding to Pseudomonas fluorescens xylanase A . Proteins Structure Function and Bioinformatics vol. 41 , ( 3 ) 362 - 373 .
Katerelos NA, Taylor MAJ, Scott M, Goodenough PW ( 1999 ) . Crystal structure of a caricain D158E mutant in complex with E‐64 . FEBS Letters vol. 392 , ( 1 ) 35 - 39 .
Leggio LL ( 1999 ) . Xylanase–oligosaccharide interactions studied by a competitive enzyme assay . Enzyme and Microbial Technology vol. 25 , ( 8-9 ) 701 - 709 .
Leggio LL, Bhat MK ( 1999 ) . High resolution structure and sequence of T. aurantiacus Xylanase I: Implications for the evolution of thermostability in family 10 xylanases and enzymes with βα‐barrel architecture . Proteins Structure Function and Bioinformatics vol. 36 , ( 3 ) 295 - 306 .
Aliwan FO, Kroon PA, Faulds CB, Williamson G ( 1999 ) . Ferulic acid esterase‐III from Aspergillus niger does not exhibit lipase activity . Journal of the Science of Food and Agriculture vol. 79 , ( 3 ) 457 - 459 .
Scott M, Pickersgill RW, Hazlewood GP, Gilbert HJ ( 1999 ) . Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa . Acta Crystallographica Section D, Structural Biology vol. 55 , ( Pt 2 ) 544 - 546 .
Pickersgill R, Smith D, Worboys K, Jenkins J ( 1999 ) . Crystallization and preliminary crystallographic analysis of the endo-polygalacturonase from Erwinia carotovora ssp. carotovora . Acta Crystallographica Section D, Structural Biology vol. 55 , ( Pt 1 ) 320 - 322 .
Harris GW, Pickersgill RW, Connerton I, Touzel J, Breton C ( 1998 ) . Structural basis of the properties of an industrially relevant thermophilic xylanase . Proteins Structure Function and Bioinformatics vol. 29 , ( 1 ) 77 - 86 .
Woo E-J, Dunwell JM, Goodenough PW ( 1998 ) . Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X‐ray crystallography . FEBS Letters vol. 437 , ( 1-2 ) 87 - 90 .
Pickersgill R, Smith D ( 1998 ) . Crystal Structure of Polygalacturonase from Erwinia carotovora ssp. carotovora * . Journal of Biological Chemistry vol. 273 , ( 38 ) 24660 - 24664 .
Pickersgill R, Harris G, Leggio LL, Mayans O ( 1998 ) . Superfamilies: the 4/7 superfamily of βα-barrel glycosidases and the right-handed parallel β-helix superfamily . Biochemical Society Transactions . vol. 26 , 190 - 197 .
Faulds CB, Aliwan FO, de Vries RP, Pickersgill RW, Visser J ( 1998 ) . Chemical and thermal stability of ferulic acid (feruloyl) esterases from Aspergillus . Progress in Biotechnology vol. 15 , 41 - 46 .
Scott M, Pickersgill RW, Hazlewood GP, Gilbert HJ ( 1998 ) . Crystallization and preliminary X-ray diffraction studies of a family 26 endo-[beta]-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa . Acta Crystallographica Section D, Structural Biology vol. 54 , ( Pt 1 ) 129 - 131 .
Jenkins J, Pickersgill R ( 1998 ) . Structure and Evolution of Parallel β-Helix Proteins . Journal of Structural Biology vol. 122 , ( 1-2 ) 236 - 246 .
Lo Leggio L, Parry NJ, Claeyssens M, Bhat MK ( 1997 ) . Crystallization and preliminary X‐ray analysis of the major endoglucanase from Thermoascus aurantiacus . Acta Crystallographica Section D, Structural Biology vol. 53 , ( 5 ) 599 - 604 .
Spurway TD, Morland C, Cooper A, Sumner I, O'Donnell AG, Pickersgill RW ( 1997 ) . Calcium Protects a Mesophilic Xylanase from Proteinase Inactivation and Thermal Unfolding* . Journal of Biological Chemistry vol. 272 , ( 28 ) 17523 - 17530 .
Mayans O, Scott M, Connerton I, Gravesen T, Benen J ( 1997 ) . Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases . Structure vol. 5 , ( 5 ) 677 - 689 .
Charnock SJ, Lakey JH, Virden R, Hughes N, Sinnott ML, Hazlewood GP ( 1997 ) . Key Residues in Subsite F Play a Critical Role in the Activity of Pseudomonas fluorescens Subspecies cellulosa Xylanase A Against Xylooligosaccharides but Not Against Highly Polymeric Substrates such as Xylan* . Journal of Biological Chemistry vol. 272 , ( 5 ) 2942 - 2951 .
Groves MR, Taylor MA, Scott M, Cummings NJ ( 1996 ) . The prosequence of procaricain forms an α-helical domain that prevents access to the substrate-binding cleft . Structure vol. 4 , ( 10 ) 1193 - 1203 .
Jenkins J, Scott M, Mayans O, Harris G, Connerton I ( 1996 ) . Crystallization and preliminary X-ray analysis of pectin lyase A from Aspergillus niger . Acta Crystallographica Section D, Structural Biology vol. 52 , ( 2 ) 402 - 404 .
Harris GW, Jenkins JA, Connerton I ( 1996 ) . Refined Crystal Structure of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens at 1.8 Å Resolution . Acta Crystallographica Section D, Structural Biology vol. 52 , ( 2 ) 393 - 401 .
Harris GW, Jenkins JA, Connerton I, Cummings N, Leggio LL, Scott M, Gilbert HJ ( 1994 ) . Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites . Structure vol. 2 , ( 11 ) 1107 - 1116 .
Taylor MAJ, Baker KC, Connerton IF, Cummings NJ, Harris GW, Henderson IMJ, Jones ST ( 1994 ) . An unequivocal example of cysteine proteinase activity affected by multiple electrostatic interactions . Protein Engineering Design and Selection vol. 7 , ( 10 ) 1267 - 1276 .
Pickersgill R, Jenkins J, Harris G ( 1994 ) . The structure of Bacillus subtilis pectate lyase in complex with calcium . Nature Structural & Molecular Biology vol. 1 , ( 10 ) 717 - 723 .
Sumner IG, Vaughan A, Eisenthal R, Pickersgill RW, Owen AJ ( 1993 ) . Kinetic analysis of papaya proteinase Ω . Biochimica et Biophysica Acta vol. 1164 , ( 3 ) 243 - 251 .
Reveil DF, Cummings NJ, Baker KC, Collins ME, Taylor MAJ, Sumner IG, Pickersgill RW ( 1993 ) . Nucleotide sequence and expression in Eschericia coli of cDNAs encoding papaya proteinase omega from Carica papaya . Gene vol. 127 , ( 2 ) 221 - 225 .
Pickersgill RW, Debeire P ( 1993 ) . Crystallization and Preliminary X-ray Analysis of a Thermophilic Bacillus Xylanase . Journal of Molecular Biology vol. 230 , ( 2 ) 664 - 666 .
Golubev AM, Neustroev KN ( 1993 ) . Crystals of β-Xylanase from Aspergillus oryzae . Journal of Molecular Biology vol. 230 , ( 2 ) 661 - 663 .
Jones ST, Ahlström P, Berendsen HJC ( 1993 ) . Molecular dynamics simulation of a phospholipase A2-substrate complex . Biochimica et Biophysica Acta vol. 1162 , ( 1-2 ) 135 - 142 .
Loris R, Steyaert J, Maes D, Lisgarten J ( 1993 ) . Corrections: Crystal Structure Determination and Refinement at 2.3-Å Resolution of the Lentil Lectin (Biochemistry (1993) 32(34) (8772–8781) (10.1021/bi00085a007)) . Biochemistry vol. 32 , ( 51 )
Pickersgill RW, Jenkins JA, Connerton I ( 1993 ) . Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens subspecies cellulosa . Journal of Molecular Biology vol. 229 , ( 1 ) 246 - 248 .
Jenkins J, Nasser W, Scott M, Pickersgill R, Vignon J-C ( 1992 ) . Crystallization and preliminary X-ray studies of the pectate lyase from Bacillus subtilis . Journal of Molecular Biology vol. 228 , ( 4 ) 1255 - 1258 .
Pickersgill RW, Harris GW ( 1992 ) . Structure of monoclinic papain at 1.60 Å resolution . Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials vol. 48 , ( 1 ) 59 - 67 .
Harris GW, Howlin B, Moss DS ( 1992 ) . The segmented anisotropic refinement of monoclinic papain by the application of the rigid‐body TLS model and comparison to bovine ribonuclease A . Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials vol. 48 , ( 1 ) 67 - 75 .
Loris R, Lisgarten J, Maes D, Körber F ( 1992 ) . Two crystal forms of the lentil lectin diffract to high resolution . Journal of Molecular Biology vol. 223 , ( 2 ) 579 - 581 .
Goodenough PW, Bhat KM, Perry BN, Pickersgill RW, Sumner IG, Verheij HM ( 1991 ) . Changes in activity of porcine phospholipase A2 brought about by charge engineering of a major structural element to alter stability . Protein Engineering Design and Selection vol. 4 , ( 8 ) 929 - 934 .
Pickersgill RW, Rizkallah P, Harris GW ( 1991 ) . Determination of the structure of papaya protease omega . Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials vol. 47 , ( 5 ) 766 - 771 .
Bhat KM, Sumner IG, Collins ME, Pickersgill RW ( 1991 ) . A novel method for the purification of porcine phospholipase A2 expressed in E.coli . Biochemical and Biophysical Research Communications vol. 176 , ( 1 ) 371 - 377 .
Pickersgill RW, Goodenough PW ( 1991 ) . Enzyme engineering . Trends in Food Science & Technology vol. 2 , 122 - 126 .
Pickersgill RW, Sumner IG, Goodenough PW ( 1989 ) . Structural and electrostatic differences between actinidin and papain account for differences in activity . Biochemical Journal vol. 257 , ( 1 ) 310 - 312 .
Pickersgill RW ( 1988 ) . A rapid method of calculating charge–charge interaction energies in proteins . Protein Engineering Design and Selection vol. 2 , ( 3 ) 247 - 248 .
Pickersgill RW, Goodenough PW, Sumner IG ( 1988 ) . The electrostatic fields in the active-site clefts of actinidin and papain . Biochemical Journal vol. 254 , ( 1 ) 235 - 238 .
Pickersgill RW ( 1987 ) . One‐dimensional disorder in spinach ribulose bisphosphate carboxylase crystals . Acta Crystallographica Section A: Foundations and advances vol. 43 , ( 4 ) 502 - 506 .
ADAMS MJ, GOVER S, PICKERSGILL RW ( 1983 ) . Structure-function relationships of NAD+- and NADP+-dependent dehydrogenases with particular reference to the three-dimensional structure of 6-phosphogluconate dehydrogenase . Biochemical Society Transactions vol. 11 , ( 4 ) 429 - 435 .
Adams MJ, Archibald IG, Carne A, Gover S, Helliwell JR ( 1983 ) . The three dimensional structure of sheep liver 6‐phosphogluconate dehydrogenase at 2.6 A resolution . The EMBO Journal vol. 2 , ( 6 ) 1009 - 1014 .
Bradley-Clarke J, Gu S, Rose R-S, Warren MJ, Pickersgill RW . Enzyme encapsulation peptides bind to the groove between tessellating subunits of the bacterial microcompartment shell .
Clarke C, Banasik M, Juodeikis R, Warren MJ, Pickersgill R . Evolutionarily divergent DUF4465 domains have a common vitamin B12-binding function .
Juodeikis R, Ulrich R, Clarke C, Banasik M, Deery E, Saalbach G, Kräutler B, Carding SR et al. . Extracellular Vesicle-Linked Vitamin B12 Acquisition via Novel Binding Proteins in Bacteroides thetaiotaomicron .
Yang Y-Y, Pickersgill RW, Fornili A . Learning fragment-based segmentation of binding sites from molecular dynamics: a proof-of-concept on cardiac myosin .
Zhang S, Gu S, Rycroft P, Ruaudel F, Delolme F, Robert X, Ballut L, Pickersgill RW et al. . Scaffolding protein GspB/OutB facilitates assembly of the Dickeya dadantii type 2 secretion system by anchoring the outer membrane secretin pore to the inner membrane and to the peptidoglycan cell wall .
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